REAP Delivers 104-Fold Enzyme Gain With AI-Robotics Platform
Updated
Updated · Nature.com · Aug 3
REAP Delivers 104-Fold Enzyme Gain With AI-Robotics Platform
1 articles · Updated · Nature.com · Aug 3
Summary
104-fold enhancement in Staphylococcus aureus Sortase A marked the top result for REAP, a closed-loop AI and robotics system for automated enzyme engineering.
RankReg—a ranking-regression learning method at REAP's core—uses experimental feedback to navigate sparse data and rugged fitness landscapes, helping the platform find useful mutations in catalytic and distal regions.
Five cycles of testing also produced a 57-fold improvement in desired activity for cytochrome P450 BM3, showing the system can progress from single mutations to productive combinations.
The Nature Communications study presents REAP as a scalable, generalizable framework for faster biocatalyst discovery and broader enzyme-enabled synthesis.